Recombinant Superfolder Green Fluorescent Protein (sfGFP)

Superfolder GFP (Green Fluorescent Protein) is an enhanced variant of the naturally occurring GFP, designed to preserve its fluorescent properties while offering greater stability and correct folding across a wider range of conditions. This makes sfGFP exceptionally valuable in scientific research, as it can be expressed in diverse environments, including high concentrations of denaturants or under stressful cellular conditions.

Manufacturing compounding

Why Choose our Recombinant sfGFP?

Our recombinant sfGFP is a versatile fluorescent marker widely used in cell biology, molecular biology, and biochemistry. Ideal for fluorescent imaging and quantification, it also serves as a reliable reference standard, helping you achieve consistent, high-quality results in your research.

  • Animal-free and free from viral contamination
  • 95% purity verified by SDS-PAGE and HPLC
  • Lyophilized powder for long-term stability and storage
  • Manufactured in an EU-GMP facility
  • Batch-to-batch consistency
  • Every batch supplied with CoA
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Core Principles Driving Quality and Global Reach

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    Fast, reliable worldwide delivery. Receive your quote within one business day and we’ll ship your order the same day you confirm.

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    Every product comes with a Certificate of Analysis for full transparency and trust.

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Product Specifications

  • Product nameRecombinant sfGFP
    SourceBacteria – Escherichia coli
    AppearanceYellow grains
    Lyophilized from buffer (50 mM NaPi; 150 mM NaCl; pH 7,2)
    Packaging sizesSizes upon request
    Purity> 95% pure based on SDS PAGE and HPLC
    SafetyNo risk of animal derived contaminations (e.g. viral particles/pathogens)
    Storage conditions and StabilityLyophilized (at least 6 months at -80 °C)
    Reconstituted (typical 1 week @ 4 °C)
    DisclaimerThis product is for R&D and manufacturing purposes only. Not for direct therapeutic use.
  • Number of Amino AcidsAA 237 (243 total, incl. Tag etc)
    Tag6x Histidine Tag
    Molecular weight27,473 Da
    Extinction Coefficient83,300 M-1 cm-1 at 488 nm
    SequenceHHHHHHSKGE ELFTGVVPIL VELDGDVNGH KFSVRGEGEG DATNGKLTLK FICTTGKLPV PWPTLVTTLT YGVQCFSRYP DHMKRHDFFK SAMPEGYVQE RTISFKDDGT YKTRAEVKFE GDTLVNRIEL KGIDFKEDGN ILGHKLEYNF NSHNVYITAD KQKNGIKANF KIRHNVEDGS VQLADHYQQN TPIGDGPVLL PDNHYLSTQS VLSKDPNEKR DHMVLLEFVT AAGITHGMDE LYK
    Referencedoi:10.1038/nbt1172

More Information About sfGFP

Want in-depth details like purity and linearity? Explore the full specifications. Download our sfGFP leaflet today and discover why it’s the right choice for your applications.

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Learn More About Us!

Basic Pharma Biotech is more than a recombinant protein shop, we are your trusted partner in protein production and purification. From lab-scale to pilot-scale, we collaborate closely, think beyond standard solutions, and deliver scalable processes tailored to your research needs. Get in contact for more information.

Recombinant sfGFP for Controlled Experimental Systems

Superfolder Green Fluorescent Protein (sfGFP) is an engineered variant of GFP designed for enhanced folding efficiency and stability. The protein exhibits strong fluorescence and maintains proper structure even when fused to poorly folding partners, making it a robust reporter for cellular and biochemical assays.

This supports fluorescent tagging, protein localization studies and quantitative assay development.

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Production and Purification

Recombinant sfGFP is produced through microbial fermentation followed by chromatography based purification. Each batch is manufactured according to predefined process parameters.

Identity and purity are verified prior to release. Manufacturing and testing are performed within our EU GMP facility.

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Applications

Recombinant sfGFP is used in:

  • Fluorescent Reporter Assays
  • Protein expression
  • Reference standard for (cell-free) sfGFP-tagged protein expressions
  • Biosensor and imaging applications
  • Analytical assay development

For projects requiring defined composition and documented production, recombinant sfGFP provides controlled input material.

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Beyond Catalog Supply

In addition to supplying recombinant sfGFP, Basic Pharma Biotech develops and manufactures custom recombinant proteins. Our in house fermentation and purification capabilities allow us to support scale up and tailored production projects.

Contact us if you require modified variants, specific buffer conditions or larger quantities.