Recombinant
TEV protease
Tobacco Etch Virus Protease (TEVp) is a highly sequence‑specific cysteine protease engineered for exceptional precision and reliability in protein processing. Recognizing the canonical ENLYFQ↓G motif, TEV protease enables clean and efficient removal of fusion tags or affinity partners, preserving the integrity, structure, and function of your protein of interest.
Produced through microbial fermentation and rigorously purified, our TEVp delivers consistent performance and optimal activity. Designed to minimize off-target cleavage, it delivers the reliability you need for confident and reproducible downstream workflows.
Why Choose Our Recombinant TEV protease
Quality You Can Trust
Whether used for sample preparation, purification workflows, assay development, or analytical studies, our TEVp provides the reliability and reproducibility needed to accelerate your research and streamline protein engineering projects.
- Optimized sequence for increased solubility and stability
- His-tagged TEV-protease for efficient post-cleavage removal via affinity purification
- 95% purity verified by SDS-PAGE and HPLC
- Lyophilized powder for long-term stability and storage
- Ready-to-use: reconstitution requires no dialysis
- Guaranteed Quality: Every Batch Supplied with CoA
Core Principles Driving Quality and Global Reach
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Worldwide Shipping
Fast, reliable worldwide delivery. Receive your quote within one business day and we’ll ship your order the same day you confirm.
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Guaranteed Quality
Every product comes with a Certificate of Analysis for full transparency and trust.
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Missing Something? Contact Us!
If you can’t find what you need or have special (product) requests, our team is ready to help.
Product Specifications
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Number of Amino Acids
1-237 (245 total, incl. His-tag etc.) Tag
N-term - 7x Histidine Tag Molecular weight
27.968 Da Extinction Coefficient
31.970 M-1 cm-1 @ 280 nm Sequence
MHHHHHHHGE SLFKGPRDYN PISSSICHLT NESDGHTTSL YGIGFGPFII TNKHLFRRNN GTLVVQSLHG VFKVKDTTTL QQHLVDGRDM IIIRMPKDFP PFPQKLKFRE PQREERICLV TTNFQTKSMS SMVSDTSCTF PSGDGIFWKH WIQTKDGQCG SPLVSTRDGF IVGIHSASNF TNTNNYFTSV PKNFMELLTN QEAQQWVSGW RLNADSVLWG GHKVFMVKPE EPFQPVKEAT QLMNE Reference
doi.org/10.1007/s00253-022-11786-5 -
Product name
Recombinant Tobacco Etch Virus (TEV) Protease Source
Bacteria – Escherichia coli Appearance
Off-white
Lyophilized from Buffer (50 mM Tris + 300 mM NaCl, pH 7,2)Packaging sizes
1000 U Purity
> 95% pure based on SDS PAGE and HPLC Safety
No risk of animal derived contaminations (e.g. viral particles/pathogens) Storage conditions and Stability
Lyophilized (at least 6 months at -20 °C)
Reconstituted (typical 1 week @ 4 °C)Disclaimer
This product is for R&D and manufacturing purposes only. Not for direct therapeutic use.
More information about our TEV protease
Want in-depth details like purity and cleavage efficacy? Explore the full specifications. Download our TEV protease leaflet today and discover why it’s the right choice for your applications
Learn More About Us!
Basic Pharma Biotech is more than a recombinant protein shop, we are your trusted partner in protein production and purification. From lab-scale to pilot-scale, we collaborate closely, think beyond standard solutions, and deliver scalable processes tailored to your research needs. Get in contact for more information.
Recombinant TEV protease
Controlled Experimental Systems
Tobacco Etch Virus protease (TEV protease, TEVp) is a highly sequence-specific cysteine protease that recognizes the ENLYFQ|G cleavage motif. Its exceptional specificity enables precise removal of affinity tags or fusion partners without affecting the target protein, resulting in clean and functional products for downstream applications.
Recombinant TEV protease expressed in Escherichia coli provides a well-defined and consistent enzyme preparation. Sequence optimization of our TEVp improves solubility and reduces autocleavage, while recombinant production ensures high purity and batch-to-batch reproducibility compared to less controlled preparations.
This supports controlled protein processing workflows, reliable tag removal and reproducible analytical and preparative results.
Production and Purification
Our recombinant TEV protease is produced through microbial fermentation and purified using chromatography-based processes to ensure high purity and consistency. Each batch is manufactured under strictly controlled process parameters.
Before release, identity and purity are fully verified and documented in a Certificate of Analysis (CoA) provided with every product.
Applications
Recombinant TEV Protease is used in:
- Removal of fusion tags, affinity tags, and solubility enhancers
- Protein purification and polishing steps
- Structural biology workflows (e.g., crystallography, cryo‑EM sample prep)
- Preparation of proteins for functional and biochemical assays
- Analytical methods requiring clean, sequence‑specific cleavage
For projects requiring precise, sequence‑defined cleavage and documented recombinant production, TEV protease offers a highly controlled and reliable processing tool.
Beyond Catalog Supply
In addition to supplying recombinant TEV protease, Basic Pharma Biotech develops and manufactures custom recombinant proteins. Our in house fermentation and purification capabilities allow us to support scale up and tailored production projects.
Contact us if you require modified variants, specific buffer conditions or larger quantities.