Recombinant Histone H3

Basic Pharma Biotech produces recombinant Histone H3 through microbial fermentation in Escherichia coli

Our recombinant Histone H3 is generated under stringent quality‑controlled conditions, ensuring consistent performance and contaminant‑free material suitable for research, diagnostic use, and early‑stage biopharmaceutical development.

We supply a single, well‑defined Histone H3 variant optimized for research, assay development, and analytical applications.

Manufacturing formulation dev

Why Choose Our Recombinant Histone H3?

Quality You Can Trust

Recombinant Histone H3, produced in E. coli, offers a high‑purity single variant compared to tissue‑derived histones, which often contain mixed forms.

Because Histone H3 is linked to cancer progression, sepsis, and inflammatory diseases, it serves as an important biomarker and research target. Our recombinant H3 is ideal for epigenetics and chromatin studies, cancer and sepsis research, antibody validation, assay development, and use as a reference standard.

  • Singe, Homogeneous Variant - No Isoform Mixture
  • Animal-Free and Free from Viral Contamination
  • 95% purity verified by SDS-PAGE and HPLC
  • Lyophilized powder for long-term stability and storage
  • Manufactured in an EU-GMP Facility
  • Every Batch Supplied with CoA
Photo Biotech Bioreactor 2

Core Principles Driving Quality and Global Reach

  • Worldwide Shipping

    Fast, reliable worldwide delivery. Receive your quote within one business day and we’ll ship your order the same day you confirm.

  • Guaranteed Quality

    Every product comes with a Certificate of Analysis for full transparency and trust.

  • Missing Something? Contact Us!

    If you can’t find what you need or have special (product) requests, our team is ready to help.

Product Specifications

  • Product name

    Recombinant Histone H3

    Source

    Bacteria – Escherichia coli

    Appearance

    White grains/powder
    Lyophilized from Buffer (25 mM HEPES + 140 mM NaCl)

    Packaging sizes

    100 µg | 1 mg

    Purity

    > 95% pure based on SDS PAGE

    Safety

    No risk of animal derived contaminations (e.g. viral particles/pathogens)

    Storage conditions and Stability

    Lyophilized (at least 6 months at -80 °C)
    Reconstituted (typical 1 week @ 4 °C)

    Disclaimer

    This product is for R&D and manufacturing purposes only. Not for direct therapeutic use.
  • Number of Amino Acids

    AA 1 – 136 (148 total, incl. Tag etc)

    Tag

    6x Histidine Tag with Thrombin Cleavage Site

    Molecular weight

    16760 Da

    Extinction Coefficient

    4470 M-1 cm-1

    Sequence

    HHHHHHLVPR GSMARTKQTA RKSTGGKAPR KQLATKAARK SAPSTGGVKK PHRYRPGTVA LREIRRYQKS TELLIRKLPF QRLVREIAQD FKTDLRFQSA AIGALQEASE AYLVGLFEDT NLCAIHAKRV TIMPKDIQLA RRIRGERA

More information about our Histone H3

Want in-depth details like purity and linearity? Explore the full specifications. Download our Histone H3 leaflet today and discover why it’s the right choice for your applications

By clicking "Download brochure" you're confirming that you agree with our Terms and Conditions

I have read and agree to the privacy policy. 
Biotech fermentor

Learn More About Us!

Basic Pharma Biotech is more than a recombinant protein shop, we are your trusted partner in protein production and purification. From lab-scale to pilot-scale, we collaborate closely, think beyond standard solutions, and deliver scalable processes tailored to your research needs. Get in contact for more information.

Recombinant Histone H3

Controlled Experimental Systems

Histone H3 is a core nucleosomal protein involved in chromatin organization and gene regulation. The protein contains residues that are subject to methylation, acetylation and phosphorylation, which regulate transcriptional activity and cellular response mechanisms.

Recombinant Histone H3 expressed in Escherichia coli provides a homogeneous protein population. In contrast to tissue derived material, recombinant production ensures defined sequence composition and avoids isoform mixtures.

This supports controlled nucleosome assembly, antibody validation and analytical testing.

Biotech pipet

Production and Purification

Recombinant Histone H3 is produced through microbial fermentation followed by chromatography based purification. Each batch is manufactured according to predefined process parameters.

Identity and purity are verified prior to release. Manufacturing and testing are performed within our EU GMP facility.

Photo Biotech Bioreactor 2

Applications

Recombinant Histone H3 is used in:

  • Chromatin and nucleosome assembly studies
  • Epigenetics research
  • Cancer and inflammation research
  • Antibody validation
  • Analytical assay development

For projects requiring defined composition and documented production, recombinant Histone H3 provides controlled input material.

Photo Biotech Filter

Beyond Catalog Supply

In addition to supplying recombinant Histone H3, Basic Pharma Biotech develops and manufactures custom recombinant proteins. Our in house fermentation and purification capabilities allow us to support scale up and tailored production projects.

Contact us if you require modified variants, specific buffer conditions or larger quantities.