sfGFP
Superfolder GFP (Green Fluorescent Protein) is an enhanced variant of the naturally occurring GFP, designed to preserve its fluorescent properties while offering greater stability and correct folding across a wider range of conditions. This makes Superfolder GFP exceptionally valuable in scientific research, as it can be expressed in diverse environments, including high concentrations of denaturants or under stressful cellular conditions.
sfGFP, a stable fluorescent protein with high intensity
sfGFP stands out from regular GFP due to its superior stability and folding efficiency under challenging conditions. Unlike regular GFP, sfGFP maintains its fluorescence even when conditions threaten the structure and fluorescence of wild-type GFP. Both sfGFP and wild-type GFP emit green fluorescence when excited by UV light, but sfGFP boasts slightly higher fluorescence intensity and enhanced photostability, making it a more effective choice for imaging applications. Are you interested in our sfGFP? Please contact us for more information.
Are you interested in our recombinant sfGFP ? Please contact us via biotech@basicpharma.nl or +31 (0)88 255 40 10!
Benefits of recombinant sfGFP from Escherichia coli
- Animal-free and free from viral contamination
- 95% purity verified by SDS-PAGE and HPLC
- Lyophilized powder for long-term stability and storage
- Ready-to-use: reconstitution requires no dialysis
- Manufactured in an EU-GMP facility
- Batch-to-batch consistency
Applications
Superfolder GFP is commonly used as a fluorescent marker in cell biology, molecular biology, and biochemistry to study protein localization, gene expression, and cellular processes.
Recombinant Protein Production Services
This sfGFP has been developed and manufactured by Basic Pharma making use of the company’s expertise in the field of protein expression, production, and engineering.
We are pleased to extend this service to new clients, providing customized solutions for the development and manufacturing of novel recombinant proteins and biopharmaceuticals.
We offer fully customized solutions for clients looking to develop novel recombinant proteins or improve the scalability and quality of their existing products. Our services cover:
- Custom gene design and strain development
- Upstream and downstream process optimization
- Small- to large-scale manufacturing
- Full analytical support and documentation
Specifications
Specifications | |
Product name | Recombinant sfGFP |
Source | Bacteria – Escherichia coli |
Appearance | Yellow grains Lyophilized from sterile filtered water |
Packaging sizes | Sizes upon request |
Purity | > 95% pure based on SDS PAGE and HPLC |
Safety | No risk of animal derived contaminations (e.g. viral particles/pathogens) |
Storage conditions and Stability | Lyophilized (at least 6 months at -80 °C) Reconstituted (typical 1 week @ 4 °C) |
Disclaimer: This product is for R&D and manufacturing purposes only. Not for direct therapeutic use. | |
| Number of Amino Acids | AA 237 (243 total, incl. Tag etc) |
| Tag | 6x Histidine Tag |
| Molecular weight | 26,781 Da |
| Extinction Coefficient | 83,300 M-1 cm-1 at 488 nm |
| Sequence | HHHHHHSKGE ELFTGVVPIL VELDGDVNGH KFSVRGEGEG DATNGKLTLK FICTTGKLPV PWPTLVTTLT YGVQCFSRYP DHMKRHDFFK SAMPEGYVQE RTISFKDDGT YKTRAEVKFE GDTLVNRIEL KGIDFKEDGN ILGHKLEYNF NSHNVYITAD KQKNGIKANF KIRHNVEDGS VQLADHYQQN TPIGDGPVLL PDNHYLSTQS VLSKDPNEKR DHMVLLEFVT AAGITHGMDE LYK |
| Reference | doi:10.1038/nbt1172 |
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