Histone H3
Histone H3 is a core component for the nucleosome, which is a complex present in chromatin and essential for DNA packaging in the nucleus. It plays a key role in gene regulation through post-translational modifications, influencing processes such as transcription, DNA repair, and cell division.
A pure, single Histone variant
Traditionally, histones are extracted from animal products like calf thymus, resulting in a mix of protein variants. However, we have developed a bacterial expression system, Escherichia coli, that is able to produce a single Histone variant. Through meticulous upstream process development (gene integration and fermentation) and refined downstream purification techniques (filtration and chromatography), we can produce a single, high-purity (>95%) histone variant, Histone H3, without relying on animal products.
Are you interested in our recombinant Histone H3? Please contact us via biotech@basicpharma.nl or +31 (0)88 255 40 10!
Benefits of recombinant Histone H3 from Escherichia coli
- Animal-free and free from viral contamination
- 95% purity verified by SDS-PAGE and HPLC
- Lyophilized powder for long-term stability and storage
- Ready-to-use: reconstitution requires no dialysis
- Manufactured in an EU-GMP facility
- Batch-to-batch consistency
Applications
In recent studies it was found that Histone H3 has a relation to the progression of sepsis. Additionally, Histone H3 was observed to be of relevance in research areas related to cancer and genetical deficiencies. Therefore, our recombinant Histone H3 is a good and safe alternative compared to the Histones obtained from animal products.
Recombinant Protein Production Services
This Histone H3 has been developed and manufactured through a collaboration between Basic Pharma (NL) and Maastricht University (NL), combining the expertise of both a research institute and an industrial company in the field of protein engineering.
We are pleased to extend this service to new clients, providing customized solutions for the development and manufacturing of novel recombinant proteins and biopharmaceuticals.
We offer fully customized solutions for clients looking to develop novel recombinant proteins or improve the scalability and quality of their existing products. Our services cover:
- Custom gene design and strain development
- Upstream and downstream process optimization
- Small- to large-scale manufacturing
- Full analytical support and documentation
Specifications
Specifications | |
Product name | Recombinant Histone H3 |
Source | Bacteria – Escherichia coli |
Appearance | White grains/powder Lyophilized from sterile filtered water |
Packaging sizes | Sizes upon request |
Purity | > 95% pure based on SDS PAGE |
Safety | No risk of animal derived contaminations (e.g. viral particles/pathogens) |
Storage conditions and Stability | Lyophilized (at least 6 months at -80 °C) Reconstituted (typical 1 week @ 4 °C) |
Disclaimer:This product is for R&D and manufacturing purposes only. Not for direct therapeutic use. | |
| Number of Amino Acids | AA 1 – 136 (148 total, incl. Tag etc) |
| Tag | 6x Histidine Tag with Thrombin Cleavage Site |
| Molecular weight | 16760 Da |
| Extinction Coefficient | 4470 M-1 cm-1 |
| Sequence |
HHHHHHLVPR GSMARTKQTA RKSTGGKAPR KQLATKAARK SAPSTGGVKK PHRYRPGTVA LREIRRYQKS TELLIRKLPF QRLVREIAQD FKTDLRFQSA AIGALQEASE AYLVGLFEDT NLCAIHAKRV TIMPKDIQLA RRIRGERA |
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